LK12-10: 3 samples (100 µg x 3)
NH2-reactive ALP: 100 µg x 3
Washing buffer: 4 ml x 1
Reaction buffer: 200 µl x 1
Storage buffer: 4 ml x 1
Filtration tube: 3 tubes
Alkaline Phosphatase Labeling Kit - NH2 is for simple and rapid preparation of alkaline phosphatase-labeled IgG for enzyme immunoassays (EIA) and immunoblotting/immunostaining. It can also be used for preparation of alkaline phosphatase-labeled antigen for competitive EIA. NH2-reactive ALP (a component of this kit) has activated ester groups, and can easily make a covalent bond with an amino group of the target molecule without any activation process. If the target is a small molecule, the conjugate can be purified with Filtration tube included in this kit. Filtration tube is also used for sample IgG in removing small molecules such as Tris buffer and amine compounds that interfere with the labeling reaction. This kit contains all of the necessary reagents for alkaline phosphatase labeling, including the storage buffer for conjugates.
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